A general approach for the development of fluorogenic probes suitable for no-wash imaging of kinases in live cells.
نویسندگان
چکیده
A general approach is presented for developing small molecule-based fluorogenic probes suitable for no-wash imaging of endogenous kinases in live cells. Probe 1, including a fluorophore-quencher system, was only "turned on" upon reacting with its target kinase Btk, and disclosed Btk's cellular location in live cells without any washing.
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عنوان ژورنال:
- Chemical communications
دوره 50 97 شماره
صفحات -
تاریخ انتشار 2014